Review



quantitative reverse transcription polymerase chain reaction qrt pcr total rna  (Vazyme Biotech Co)


Bioz Verified Symbol Vazyme Biotech Co is a verified supplier
Bioz Manufacturer Symbol Vazyme Biotech Co manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Vazyme Biotech Co quantitative reverse transcription polymerase chain reaction qrt pcr total rna
    Quantitative Reverse Transcription Polymerase Chain Reaction Qrt Pcr Total Rna, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 99/100, based on 3015 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rna+reverse+transcription/FreeZol+Reagent/pm42894231-384-3-23
    Average 99 stars, based on 3015 article reviews
    quantitative reverse transcription polymerase chain reaction qrt pcr total rna - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Proteomic profiling and molecular reclassification of high-grade serous ovarian cancer identifies prognostic subtypes and immunotherapy biomarkers.
    Article Snippet: .. HiScript III RT SuperMix for qPCR (+gDNA wiper) (Vazyme Biotech, R323-01) and ChamQ Universal SYBR qPCR Master Mix (Nanjing Vazyme Biotech, Q711-02) were used for the RNA reverse transcription and PCR analysis. ..

    Article Title: Knockdown of KIF15 suppresses proliferation of prostate cancer cells and induces apoptosis through PI3K/Akt signaling pathway
    Article Snippet: The total RNA was extracted according to Sigma’s Trizol operating instructions. .. RNA reverse transcription was performed and cDNA was obtained according to Vazyme Hiscript QRT Supermix for qPCR (+gDNA WIPER) operating instructions. ..

    Article Title: Identifying key genes related to the peritubular capillary rarefaction in renal interstitial fibrosis by bioinformatics
    Article Snippet: .. Subsequently, RNA reverse transcription was performed using HiScript III RT SuperMix (Vazyme, Nanjing, China). qPCR was carried out using the Tap Pro Universal SYBR qPCR Master Mix (Vazyme, Nanjing, China). mRNA expression levels were detected on StepOne software v2.3. ..

    Article Title: Proteomic profiling and molecular reclassification of high-grade serous ovarian cancer identifies prognostic subtypes and immunotherapy biomarkers
    Article Snippet: .. HiScript III RT SuperMix for qPCR (+gDNA wiper) (Vazyme Biotech, R323-01) and ChamQ Universal SYBR qPCR Master Mix (Nanjing Vazyme Biotech, Q711-02) were used for the RNA reverse transcription and PCR analysis. ..

    Article Title: miR-181b-1-3p affects the proliferation and differentiation of chondrocytes in TD broilers through the WIF1/Wnt/β-catenin pathway.
    Article Snippet: Thiram is a plant fungicide, its excessive use has exceeded the required environmental standards.. It causes tibial dyschondroplasia (TD) in broilers which is a common metabolic disease that affects the growth plate of tibia bone.. It has been studied that many microRNAs (miRNAs) are involved in the differentiation of chondrocytes however, their specific roles and mechanisms have not been fully investigated.

    Reverse Transcription:

    Article Title: Proteomic profiling and molecular reclassification of high-grade serous ovarian cancer identifies prognostic subtypes and immunotherapy biomarkers.
    Article Snippet: .. HiScript III RT SuperMix for qPCR (+gDNA wiper) (Vazyme Biotech, R323-01) and ChamQ Universal SYBR qPCR Master Mix (Nanjing Vazyme Biotech, Q711-02) were used for the RNA reverse transcription and PCR analysis. ..

    Article Title: Tangzhiqing decoction attenuates cognitive dysfunction of mice with type 2 diabetes by regulating AMPK/mTOR autophagy signaling pathway.
    Article Snippet: Ethnopharmacological relevance: Tangzhiqing decoction (TZQD) is an effective prescription developed by Jiangsu Province Hospital of Chinese Medicine for the treatment of diabetes mellitus (DM) and its complications, which has a clear cerebral protective effect on mice with diabetic cognitive dysfunction, but its specific mechanism has not been well elucidated.. Aims of the study: This study aims to verify the protection of TZQD on cognitive function in mice with type 2 diabetes mellitus (T2DM) and explore the possible underlying mechanisms.. Materials and methods: Six active ingredients in TZQD were detected using high-performance liquid chromatography analysis.

    Article Title: Knockdown of KIF15 suppresses proliferation of prostate cancer cells and induces apoptosis through PI3K/Akt signaling pathway
    Article Snippet: The total RNA was extracted according to Sigma’s Trizol operating instructions. .. RNA reverse transcription was performed and cDNA was obtained according to Vazyme Hiscript QRT Supermix for qPCR (+gDNA WIPER) operating instructions. ..

    Article Title: Identifying key genes related to the peritubular capillary rarefaction in renal interstitial fibrosis by bioinformatics
    Article Snippet: .. Subsequently, RNA reverse transcription was performed using HiScript III RT SuperMix (Vazyme, Nanjing, China). qPCR was carried out using the Tap Pro Universal SYBR qPCR Master Mix (Vazyme, Nanjing, China). mRNA expression levels were detected on StepOne software v2.3. ..

    Article Title: Proteomic profiling and molecular reclassification of high-grade serous ovarian cancer identifies prognostic subtypes and immunotherapy biomarkers
    Article Snippet: .. HiScript III RT SuperMix for qPCR (+gDNA wiper) (Vazyme Biotech, R323-01) and ChamQ Universal SYBR qPCR Master Mix (Nanjing Vazyme Biotech, Q711-02) were used for the RNA reverse transcription and PCR analysis. ..

    Article Title: miR-181b-1-3p affects the proliferation and differentiation of chondrocytes in TD broilers through the WIF1/Wnt/β-catenin pathway.
    Article Snippet: Thiram is a plant fungicide, its excessive use has exceeded the required environmental standards.. It causes tibial dyschondroplasia (TD) in broilers which is a common metabolic disease that affects the growth plate of tibia bone.. It has been studied that many microRNAs (miRNAs) are involved in the differentiation of chondrocytes however, their specific roles and mechanisms have not been fully investigated.

    Article Title: Silencing HOXC13 exerts anti-prostate cancer effects by inducing DNA damage and activating cGAS/STING/IRF3 pathway.
    Article Snippet: Total RNA was extracted from cells and tissues using Trizol reagent (BioTeke, China). .. After determining the concentration and purity of RNA samples, RNA reverse transcription was performed according to the steps of reverse transcription kit (Vazyme, China). .. The synthetic cDNAs were used as template for fluorescence detection using SYBR Green qPCR detection kit (Biosharp, China).

    Article Title: Silencing HOXC13 exerts anti-prostate cancer effects by inducing DNA damage and activating cGAS/STING/IRF3 pathway
    Article Snippet: Total RNA was extracted from cells and tissues using Trizol reagent (BioTeke, China). .. After determining the concentration and purity of RNA samples, RNA reverse transcription was performed according to the steps of reverse transcription kit (Vazyme, China). .. The synthetic cDNAs were used as template for fluorescence detection using SYBR Green qPCR detection kit (Biosharp, China).

    Polymerase Chain Reaction:

    Article Title: Proteomic profiling and molecular reclassification of high-grade serous ovarian cancer identifies prognostic subtypes and immunotherapy biomarkers.
    Article Snippet: .. HiScript III RT SuperMix for qPCR (+gDNA wiper) (Vazyme Biotech, R323-01) and ChamQ Universal SYBR qPCR Master Mix (Nanjing Vazyme Biotech, Q711-02) were used for the RNA reverse transcription and PCR analysis. ..

    Article Title: Proteomic profiling and molecular reclassification of high-grade serous ovarian cancer identifies prognostic subtypes and immunotherapy biomarkers
    Article Snippet: .. HiScript III RT SuperMix for qPCR (+gDNA wiper) (Vazyme Biotech, R323-01) and ChamQ Universal SYBR qPCR Master Mix (Nanjing Vazyme Biotech, Q711-02) were used for the RNA reverse transcription and PCR analysis. ..

    RNA Extraction:

    Article Title: Tangzhiqing decoction attenuates cognitive dysfunction of mice with type 2 diabetes by regulating AMPK/mTOR autophagy signaling pathway.
    Article Snippet: Ethnopharmacological relevance: Tangzhiqing decoction (TZQD) is an effective prescription developed by Jiangsu Province Hospital of Chinese Medicine for the treatment of diabetes mellitus (DM) and its complications, which has a clear cerebral protective effect on mice with diabetic cognitive dysfunction, but its specific mechanism has not been well elucidated.. Aims of the study: This study aims to verify the protection of TZQD on cognitive function in mice with type 2 diabetes mellitus (T2DM) and explore the possible underlying mechanisms.. Materials and methods: Six active ingredients in TZQD were detected using high-performance liquid chromatography analysis.

    Amplification:

    Article Title: Tangzhiqing decoction attenuates cognitive dysfunction of mice with type 2 diabetes by regulating AMPK/mTOR autophagy signaling pathway.
    Article Snippet: Ethnopharmacological relevance: Tangzhiqing decoction (TZQD) is an effective prescription developed by Jiangsu Province Hospital of Chinese Medicine for the treatment of diabetes mellitus (DM) and its complications, which has a clear cerebral protective effect on mice with diabetic cognitive dysfunction, but its specific mechanism has not been well elucidated.. Aims of the study: This study aims to verify the protection of TZQD on cognitive function in mice with type 2 diabetes mellitus (T2DM) and explore the possible underlying mechanisms.. Materials and methods: Six active ingredients in TZQD were detected using high-performance liquid chromatography analysis.

    Expressing:

    Article Title: Identifying key genes related to the peritubular capillary rarefaction in renal interstitial fibrosis by bioinformatics
    Article Snippet: .. Subsequently, RNA reverse transcription was performed using HiScript III RT SuperMix (Vazyme, Nanjing, China). qPCR was carried out using the Tap Pro Universal SYBR qPCR Master Mix (Vazyme, Nanjing, China). mRNA expression levels were detected on StepOne software v2.3. ..

    Software:

    Article Title: Identifying key genes related to the peritubular capillary rarefaction in renal interstitial fibrosis by bioinformatics
    Article Snippet: .. Subsequently, RNA reverse transcription was performed using HiScript III RT SuperMix (Vazyme, Nanjing, China). qPCR was carried out using the Tap Pro Universal SYBR qPCR Master Mix (Vazyme, Nanjing, China). mRNA expression levels were detected on StepOne software v2.3. ..

    Concentration Assay:

    Article Title: Silencing HOXC13 exerts anti-prostate cancer effects by inducing DNA damage and activating cGAS/STING/IRF3 pathway.
    Article Snippet: Total RNA was extracted from cells and tissues using Trizol reagent (BioTeke, China). .. After determining the concentration and purity of RNA samples, RNA reverse transcription was performed according to the steps of reverse transcription kit (Vazyme, China). .. The synthetic cDNAs were used as template for fluorescence detection using SYBR Green qPCR detection kit (Biosharp, China).

    Article Title: Silencing HOXC13 exerts anti-prostate cancer effects by inducing DNA damage and activating cGAS/STING/IRF3 pathway
    Article Snippet: Total RNA was extracted from cells and tissues using Trizol reagent (BioTeke, China). .. After determining the concentration and purity of RNA samples, RNA reverse transcription was performed according to the steps of reverse transcription kit (Vazyme, China). .. The synthetic cDNAs were used as template for fluorescence detection using SYBR Green qPCR detection kit (Biosharp, China).



    Similar Products

    99
    Vazyme Biotech Co quantitative reverse transcription polymerase chain reaction qrt pcr total rna
    Quantitative Reverse Transcription Polymerase Chain Reaction Qrt Pcr Total Rna, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rna+reverse+transcription/FreeZol+Reagent/pm42894231-384-3-23
    Average 99 stars, based on 1 article reviews
    quantitative reverse transcription polymerase chain reaction qrt pcr total rna - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    96
    TaKaRa ecodry premix reverse transcription kit
    Ecodry Premix Reverse Transcription Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rna+reverse+transcription/RNA+to+cDNA+EcoDry+Premix/pmc13650181-354-8-13
    Average 96 stars, based on 1 article reviews
    ecodry premix reverse transcription kit - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    99
    TaKaRa reverse transcription quantitative polymerase chain reaction rt qpcr total rna
    Reverse Transcription Quantitative Polymerase Chain Reaction Rt Qpcr Total Rna, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rna+reverse+transcription/RNAiso+Plus/pm42844289-336-0-13
    Average 99 stars, based on 1 article reviews
    reverse transcription quantitative polymerase chain reaction rt qpcr total rna - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    97
    Vazyme Biotech Co o ctober 2026 quantitative reverse transcription polymerase chain reaction rt qpcr rna
    O Ctober 2026 Quantitative Reverse Transcription Polymerase Chain Reaction Rt Qpcr Rna, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rna+reverse+transcription/FastPure+Universal+Plant+Total+RNA+Isolation+Kit/pm41779674-369-17-46
    Average 97 stars, based on 1 article reviews
    o ctober 2026 quantitative reverse transcription polymerase chain reaction rt qpcr rna - by Bioz Stars, 2026-10
    97/100 stars
      Buy from Supplier

    99
    TaKaRa reverse transcription quantitative pcr rt qpcr total rna
    Reverse Transcription Quantitative Pcr Rt Qpcr Total Rna, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rna+reverse+transcription/RNAiso+Plus/pm41703985-53-3-19
    Average 99 stars, based on 1 article reviews
    reverse transcription quantitative pcr rt qpcr total rna - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    86
    Molecular Dynamics Inc solid tumors rna seq rna sequencing moco v2 momentum contrast v2 rt qpcr reverse transcription quantitative polymerase chain reaction cv coefficient
    Solid Tumors Rna Seq Rna Sequencing Moco V2 Momentum Contrast V2 Rt Qpcr Reverse Transcription Quantitative Polymerase Chain Reaction Cv Coefficient, supplied by Molecular Dynamics Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rna+reverse+transcription/cell+rna+sequencing+single/pm42277915-283-33-54
    Average 86 stars, based on 1 article reviews
    solid tumors rna seq rna sequencing moco v2 momentum contrast v2 rt qpcr reverse transcription quantitative polymerase chain reaction cv coefficient - by Bioz Stars, 2026-10
    86/100 stars
      Buy from Supplier

    99
    TaKaRa rna reverse transcription
    Rna Reverse Transcription, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rna+reverse+transcription/PrimeScript+RT+Master+Mix/pm42100872-272-0-11
    Average 99 stars, based on 1 article reviews
    rna reverse transcription - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    99
    Vazyme Biotech Co rna reverse transcription
    Rna Reverse Transcription, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rna+reverse+transcription/ChamQ+Universal+SYBR+qPCR+Master+Mix/pmc13101710-131-26-19
    Average 99 stars, based on 1 article reviews
    rna reverse transcription - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    86
    Yeasen Biotechnology rna reverse transcription kit
    <t>RNA-sequencing</t> data revealed that hOsteo4-E9 cells not only share some similarity with MLO-Y4 cells, but also have distinct <t>transcription</t> profiles in response to FSS. (A) PCA plot of gene expression variability of human hOsteo4-E9, murine MLO-Y4, and murine long bone-derived osteocytes; (B) Venn diagram of detected genes in hOsteo4-E9 and MLO-Y4 cells; (C) Venn diagram of detected genes in 4 experimental groups, that is, E9_F, E9_S, Y4_F, and Y4_S; (D-F) top 15 of KEGG enriched pathways in all detected genes, hOsteo4-E9 specific and MLO-Y4 specific genes; (G) clustering heat map highlighted the variations of FA-associated gene expression patterns between 4 experimental groups; (H-M) qPCR validation of gene expression of integrin αν, integrin α5, integrin α3, integrin β5, integrin β7, and EMP1 in 4 experimental groups. N = 3 for each group. Results are expressed as mean ± SD. n.s. p > .05; * p < .05; ** p < .01; *** p < .001.
    Rna Reverse Transcription Kit, supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rna+reverse+transcription/kit+reverse+transcription/pmc13050506-87-12-18
    Average 86 stars, based on 1 article reviews
    rna reverse transcription kit - by Bioz Stars, 2026-10
    86/100 stars
      Buy from Supplier

    86
    Yesen Biotech real time quantitative reverse transcription polymerase chain reaction rna
    <t>RNA-sequencing</t> data revealed that hOsteo4-E9 cells not only share some similarity with MLO-Y4 cells, but also have distinct <t>transcription</t> profiles in response to FSS. (A) PCA plot of gene expression variability of human hOsteo4-E9, murine MLO-Y4, and murine long bone-derived osteocytes; (B) Venn diagram of detected genes in hOsteo4-E9 and MLO-Y4 cells; (C) Venn diagram of detected genes in 4 experimental groups, that is, E9_F, E9_S, Y4_F, and Y4_S; (D-F) top 15 of KEGG enriched pathways in all detected genes, hOsteo4-E9 specific and MLO-Y4 specific genes; (G) clustering heat map highlighted the variations of FA-associated gene expression patterns between 4 experimental groups; (H-M) qPCR validation of gene expression of integrin αν, integrin α5, integrin α3, integrin β5, integrin β7, and EMP1 in 4 experimental groups. N = 3 for each group. Results are expressed as mean ± SD. n.s. p > .05; * p < .05; ** p < .01; *** p < .001.
    Real Time Quantitative Reverse Transcription Polymerase Chain Reaction Rna, supplied by Yesen Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rna+reverse+transcription/kit+reverse+transcription/pm42069297-102-0-39
    Average 86 stars, based on 1 article reviews
    real time quantitative reverse transcription polymerase chain reaction rna - by Bioz Stars, 2026-10
    86/100 stars
      Buy from Supplier

    Image Search Results


    RNA-sequencing data revealed that hOsteo4-E9 cells not only share some similarity with MLO-Y4 cells, but also have distinct transcription profiles in response to FSS. (A) PCA plot of gene expression variability of human hOsteo4-E9, murine MLO-Y4, and murine long bone-derived osteocytes; (B) Venn diagram of detected genes in hOsteo4-E9 and MLO-Y4 cells; (C) Venn diagram of detected genes in 4 experimental groups, that is, E9_F, E9_S, Y4_F, and Y4_S; (D-F) top 15 of KEGG enriched pathways in all detected genes, hOsteo4-E9 specific and MLO-Y4 specific genes; (G) clustering heat map highlighted the variations of FA-associated gene expression patterns between 4 experimental groups; (H-M) qPCR validation of gene expression of integrin αν, integrin α5, integrin α3, integrin β5, integrin β7, and EMP1 in 4 experimental groups. N = 3 for each group. Results are expressed as mean ± SD. n.s. p > .05; * p < .05; ** p < .01; *** p < .001.

    Journal: JBMR Plus

    Article Title: Establishment and characterization of a human pre-osteocyte cell line: hOsteo4-E9

    doi: 10.1093/jbmrpl/ziaf163

    Figure Lengend Snippet: RNA-sequencing data revealed that hOsteo4-E9 cells not only share some similarity with MLO-Y4 cells, but also have distinct transcription profiles in response to FSS. (A) PCA plot of gene expression variability of human hOsteo4-E9, murine MLO-Y4, and murine long bone-derived osteocytes; (B) Venn diagram of detected genes in hOsteo4-E9 and MLO-Y4 cells; (C) Venn diagram of detected genes in 4 experimental groups, that is, E9_F, E9_S, Y4_F, and Y4_S; (D-F) top 15 of KEGG enriched pathways in all detected genes, hOsteo4-E9 specific and MLO-Y4 specific genes; (G) clustering heat map highlighted the variations of FA-associated gene expression patterns between 4 experimental groups; (H-M) qPCR validation of gene expression of integrin αν, integrin α5, integrin α3, integrin β5, integrin β7, and EMP1 in 4 experimental groups. N = 3 for each group. Results are expressed as mean ± SD. n.s. p > .05; * p < .05; ** p < .01; *** p < .001.

    Article Snippet: Next, 1 μg of total RNA was reverse-transcribed into cDNA using an RNA reverse transcription kit (Cat# 11141ES60, Yeasen, Shanghai, China).

    Techniques: RNA Sequencing, Gene Expression, Derivative Assay, Biomarker Discovery